AFO Chromatography Domain Ontology (REC/2021/03)

A piece of apparatus that has the form of a tube with a diameter of less than a millimeter and hosts the stationary bed in chromatography. [CHMO]

A chromatography column used for analysis. [Allotrope]

A piece of apparatus that is planar and is or hosts the stationary bed in chromatography. [CHMO]

A chromatography detector is a detector that is used in chromatography as component of a chromatography system. [Allotrope]

A chromatography column where the stationary phase is a strong anion exchanger. [CHMO]

A chromatography column where the stationary phase is a weak anion exchanger. [CHMO]

A chromatography column where the stationary phase is a strong cation exchanger. [CHMO]

A short protective chromatography column used to protect the main analytical column from damaging impurities that are injected into the instrument. [Allotrope]

A piece of apparatus, consisting of an eluent supply system, an injection or sampling system, a column containing a swollen gel, a detector and a data acquisition/processing system, that is used to carry out chromatographic separations. [CHMO]

A chromatography column where the stationary phase is a cation exchanger. [CHMO]

A chromatography column where the stationary phase is an anion exchanger. [CHMO]

A capillary electrophoresis column is a type of column that is used in capillary electrophoresis. [Allotrope]

A chromatography column in which either the inner tube wall or a liquid or active solid held stationary on the tube wall acts as the stationary phase and there is an open, unrestricted path for the mobile phase. [CHMO]

A chromatography column that contains a solid packing. [CHMO]

An array column is a column that contains multiple columns at the same time. [Allotrope]

A chromatography column where the stationary phase is a weak cation exchanger. [CHMO]

A chromatography column where the stationary phase is an ion exchanger. [CHMO]

A spin column is a column used for concentration of biological samples containing membranes for ultrafiltration. The solid phase is made up of silica to which the biological sample binds. [Allotrope]

A device in which the sample is first introduced into a chamber (loop), temporarily isolated from the mobile phase system by valves, which can be switched to make an instantaneous diversion of the mobile phase stream through the chamber to carry the sample to the column. [IUPAC]

A device which directly introduces the sample into the mobile-phase stream. [IUPAC]

A device which directly introduces the sample into the mobile-phase stream. [IUPAC]

A column compartment is a compartment in a chromatography system where the columns are held. [Allotrope]

A monolithic columns is a chromatography column where the stationary phase synthesized in place as a single lattice structure in the column. [Allotrope]

A chromatography data system is a data system that controls a chromatography system and processes data participating in a chromatography run. [Allotrope]

To deliver material is the function to transfer a material to or into a destination. [Allotrope]

To deliver mobile phase is the function to deliver material that is in a mobile phase role. [Allotrope]

A material separation function which is realized by chromatography. [Allotrope]

A chromatographic packing is a solid heterogeneous mixture consisting of a substrate and stationary phase used in a chromatographic separation. [Allotrope]

Chromatography in which separation is based mainly on differences between the solubility of the sample components in the stationary phase (gas chromatography), or on differences between the solubilities of the components in the mobile and stationary phases (liquid chromatography). [CHMO]

A chromatography method where the stationary bed is within a tube (of standard length 25 cm). The particles of the solid stationary phase or support coated with a liquid stationary phase may fill the whole inside volume of the tube (packed column) or be concentrated on or along the inside tube wall leaving an open, unrestricted path for the mobile phase in the middle part of the tube (open-tubular column). [CHMO]

Chromatography in which separation is based mainly on differences between the adsorption affinities of the sample components for the surface of an active solid. [CHMO]

A separation method where the components are distributed between two phases, one of which is stationary and polar, while the other is non-polar and moves in a definite direction. [CHMO]

Process of loading a sample into a chromatography system. [Allotrope]

A separation method where the components (in this case stereoisomers) are distributed between two chiral phases, one of which is stationary, while the other moves in a definite direction. [CHMO]

A chromatography method where the stationary phase is present as or on a plane. [CHMO]

Chromatography data processing is a data transformation on chromatograms. [Allotrope]

Chromatography where the mobile phase contains a compound (the displacer) more strongly retained than the components of the sample under examination. The sample is fed into the system as a finite slug. [CHMO]

A separation method where the components are distributed between two phases, one of which is stationary and non-polar, while the other is polar and moves in a definite direction. [CHMO]

Headspace chromatography is a type of chromatography that uses the gaseous headspace above a sample for analysis because very light volatiles can be efficiently partitioned as they diffuse to the gas volume above. [Allotrope]

Mixed-mode chromatography (MMC), or multimodal chromatography, refers to chromatographic methods that utilize more than one form of interaction between the stationary phase and analytes in order to achieve separation. [Wikipedia]

A chromatography method where the separation is based on the adsorption to membranes. [Allotrope]

Mobile phase delivery is a process performed by a chromatography system in which the mobile phase is mixed and transported through the instrument for separation and detection. [Allotrope]

The relative retention calculation is a process of performing the mathematical ratio quantification of the time a substance and a standard that remain in the stationary phase of a chromatography process. [Allotrope]

Constant pressure mobile phase delivery is a mobile phase delivery process whereby the mobile phase is delivered by maintaining a constant pressure of the mobile phase. [Allotrope]

Constant flow rate mobile phase delivery is a mobile phase delivery process whereby the mobile phase is delivered by maintaining a constant flow rate of the mobile phase. [Allotrope]

The length of a chromatography column bed. [Allotrope]

Column inner diameter is the inner diameter of a column. [Allotrope]

Relative retention time is a ratio quality of the adjusted or net retention time of a component relative to that of a standard, obtained under identical conditions. [Allotrope]

The hold-up volume is the volume of the mobile phase required to elute a component the concentration of which in the stationary phase is negligible compared to that in the mobile phase. In other words, this component is not retained at all by the stationary phase. Thus, the hold-up volume is equal to the retention volume of an unretained compound. [IUPAC]

A bonded phase is the structure quality of a material that is covalently bonded to a substrate. [Allotrope]

A chromatography peak is a peak that results from a detector's response to the presence of an analyte in the mobile phase eluting from a column in chromatography. [Allotrope]

A plot of detector signal (e.g. ion count or absorbance) vs. retention time obtained from a column chromatography experiment where the mobile phase is a gas. [CHMO]

A mass chromatogram is a distribution function of a mass detector signal versus time of acquisition or versus volume of analyte. [Allotrope]

An electromagnetic radiation chromatogram is a distribution function of a electromagnetic radiation detector signal versus time of acquisition or versus volume of analyte. [Allotrope]

A plot of detector signal (e.g. ion count or absorbance) vs. retention time obtained from a chromatography experiment where the separation is caused by exclusion effects within the stationary phase and where the stationary phase is a swollen gel. [CHMO]

A ion chromatogram is a distribution function of a ion current signal of a mass detector versus time of acquisition or versus volume of analyte. [Allotrope]

A plot of detector signal (e.g. ion count or absorbance) vs. retention time obtained from a chromatography experiment where the separation is caused by differences in molecular size. [CHMO]

An unretained peak is a peak which represents the detection result of a material entity which was not retained by a chromatography column. [Allotrope]

Retention time is a chromatography peak facet that indicates the elapsed time between the injection of the sample and the maximum detector response attributed to the peak (analyte band). [Allotrope]

A chromatography peak facet that denotes the retention time of the peak relative to some other specified reference peak. [Allotrope]

A relative retention time that quantitates the ratio of the retention time of the peak relative to some other specified reference peak. [Allotrope]

A relative retention time that quantitates the ratio of the adjusted retention time (corrected for hold-up time) of the peak relative to the adjusted retention time of some reference peak. [Allotrope]

A chromatography peak facet is a facet of a chromatography peak. [Allotrope]

The portion of the chromatogram recording the detector response when only the mobile phase emerges from the column. [IUPAC]

A chromatographic peak facet that quantitates the degree of separation between two peaks of a chromatogram. [Allotrope]

Chromatographic peak resolution that is calculated by the USP <621> method using peak widths measured at the baseline. [Allotrope]

Chromatographic peak resolution that is calculated using peak widths measured at the half height. [Allotrope]

Chromatographic peak resolution that is calculated based on peak widths derived from the second statistical moment (peak variance). [Allotrope]

The capacity factor is a chromatography peak facet that is a measure of the time the sample component resides in the stationary phase relative to the time it resides in the mobile phase: it expresses how much longer a sample component is retarded by the stationary phase than it would take to travel through the column with the velocity of the mobile phase. [IUPAC]

A chromatography peak facet that denotes the ratio of the peak's capacity factor to that of another specified peak. [Allotrope]

A chromatography peak facet that denotes the ratio of the peak's capacity factor to that of another specified peak. [Allotrope]

A quantitative asymmetry factor using 5% peak height. [Allotrope]

A quantitative asymmetry factor using the third statistical moment calculated from the peak. [Allotrope]

A chromatography peak facet that measures the amount of band broadening that occurs during the separation and thus the efficiency of the chromatographic system. [Allotrope]

The number of theoretical plates calculated based on the peak width measured at the baseline. [Allotrope]

The number of theoretical plates calculated based on the peak width measured at half height. [Allotrope]

A parameter specification specific to the sampler device in the chromatography system. [Allotrope]

An autosampler parameter specification that specifies the volume of sample the autosampler is to inject into the mobile phase for separation. [Allotrope]

A parameter specification specific to the pump device in the chromatography system. [Allotrope]

A pump parameter specification that specifies the mobile phase ratio in the eluent. [Allotrope]

A pump parameter specification that specifies the total flow rate of the combined mobile phases (eluent). [Allotrope]

Washing repetition setting is a setting that sets the number of repetitions of the washing. [Allotrope]

Washing duration setting is a setting that sets the duration of the washing. [Allotrope]

Washing vial position setting is a setting that sets the position of the washing vial. [Allotrope]

Washing solvent type is a setting that sets the type of washing solvent to use. [Allotrope]

An autosampler temperature setting is a setting that sets the temperature within the autosampler. [Allotrope]

Maximum flow rate change setting is a control setting that sets the maximum rate at which the pump is allowed to change the % of solvents being flowed. [Allotrope]

A parameter specification specific to a chromatography system. [Allotrope]

Autosampler injection trigger enable setting is a setting that activates or deactivates triggering of injection based on a specified temperature range. [Allotrope]

Cooler (with range) can be enabled in order to trigger injection once autosampler temperature has reached a certain value. Enabling the setting also involves activation of the cooler.

Autosampler injection trigger temperature setting is a setting that specifies the temperature range for triggering injection. [Allotrope]

Mobile phase delivery duration setting is a chromatography system setting that sets the total duration of mobile phase delivery. [Allotrope]

Frit size is a column specification that indicates the nominal size of pores in a frit designed to exclude particles larger than the specified size. [Allotrope]

Baseline at start of integration setting is a data processing setting that sets the ordinate value at the start of the integration baseline to the the value of the signal at the time indicated. [Allotrope]

Peak end setting is a data processing setting that sets the peak end to the value of the abscissa at the time indicated. [Allotrope]

Baseline boundary setting is a data processing setting that sets the ordinate values at the boundaries of the baseline of integration to the actual signal values at the peak start and peak end. [Allotrope]

Integration with peak detection enabled setting is a data processing setting that allows the integration process to detect peaks for the time period specified. [Allotrope]

Negative peak detection enabled is a data processing setting that allows peaks to be detected below the baseline for the time specified. [Allotrope]

Area threshold for peak integration setting is a data processing setting that sets a minimum or maximum area for a peak to be included in the integrated peak list. [Allotrope]

Height threshold for peak integration setting is a data processing setting that sets a minimum or maximum height for a peak to be included in the integrated peak list. [Allotrope]

Expected average peak width setting is a data processing setting that indicates a typical peak width for the time period specified. [Allotrope]

Shoulder detection enabled setting is a data processing setting that allows the integration process to interpolate a baseline for the smaller peak of overlapping peaks for the time period specified. [Allotrope]

Signal-to-noise ratio (USP621) is a signal-to-noise ratio (peak-to-peak) where the noise is calculated over a period that is a minimum of 5 times the peak width. [Allotrope]

Signal-to-noise ratio (EP2.2.46) is a signal-to-noise ratio (peak-to-peak) where the noise is calculated over a period that is a minimum of 20 times the peak width. [Allotrope]

The retention time window lower offset is the extent of the retention time window in time units below the target retention time. [PSI/MS]

The lower and upper offsets may be asymmetric about the target time. [PSI/MS]

The retention time window upper offset is the extent of the retention time window in time units above the target retention time. [PSI/MS]

The lower and upper offsets may be asymmetric about the target time. [PSI/MS]

The retention time window width it the full width of a retention time window for a chromatographic peak. [PSI/MS]

A chromatography data system method is a plan specification that specifies how a process is to be executed on a chromatography data system. [Allotrope]

A column brand name is a brand name of a chromatography column that can be based on the brand name of the substrate and its chemistry modification. [Allotrope]

A chromatography column classification is a classification datum that classifies a chromatography column by the stationary phase and chromatographic packing used. [Allotrope]

A chromatography column length (datum) is a length datum that quantifies the length of a chromatography column. [Allotrope]

A column inner diameter (datum) is an inner diameter quantifies the inner diameter of a column. [Allotrope]

Mobile phase is the chromatographic phase role of a fluid material entity in a chromatography process to act as the fluid that percolates through or along the stationary bed in chromatography. [Allotrope, CHMO]

A stationary phase is the chromatographic phase role of a solid, gel or liquid material that is the stationary component in chromatography. [Allotrope]

One of the portions of material between which the components to be separated are distributed in chromatography. [CHMO]

Gaseous mobile phase is the mobile phase role of a gaseous material. [Allotrope]

Supercritical mobile phase is the mobile phase role of a supercritical fluid material. [Allotrope]

Effluent is a mobile phase eluting from a column. [Allotrope]

A mobile phase that is analyte material coming from the chromatograph. [Allotrope]

Eluent is a mobile phase entering a column to perform a separation of material. [Allotrope]

A capillary inner coating role is a coating role of a material that is a coating within a chromatography column. [Allotrope]

An endcapping role is a supporting material role with the purpose of preventing chromatographic analytes from interacting with the substrate of the stationary phase. [Allotrope]